Current CapabilitiesAD v PS RUO Assay Biomarker Analysis
AD v PS RUO Assay
Epidermal skin samples were non-invasively collected from the lesional skin of the patients (participants in IRB approved protocol) with moderate to severe AD or moderate to severe PS. RNA was then isolated and analyzed by quantitative real-time PCR for expression of the following biomarkers:
- AD associated biomarkers: IL-4R, IL-13, CCL17/TARC, CCL26/Eotaxin 3
- PS associated biomarkers: IL-17A, IL-22, IL-23A, NOS2, CXCL9, CXCL10
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Using a machine learning approach, we identified a unique ratio that stratified AD versus PS subjects. The algorithm uses a combination of expression from IL-17A and NOS2 compared to the combined expression of CCL17 and IL-13.4
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